Catalog Number - 40031-15P

40031-15P Cogent UDA™ (weak cation exchange, WCX) HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), offering unique mixed‑mode selectivity that combines reversed phase, ion exchange, and HILIC interactions in one highly robust stationary phase.

The UDA™ ligand (Undecanoic Acid) consists of an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, allowing multiple retention mechanisms:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (active at pH ≥ 6)

• HILIC (polar interaction capability)

This multi‑mode capability provides superior selectivity for basic, highly polar, and ionizable compounds, making it especially effective for complex matrices and challenging separations where traditional C18 columns fall short.

The TYPE‑C silica hydride base delivers critical performance benefits:

• Direct silicon–carbon bonding for exceptional chemical stability

• Operates at very low pH (<2) without degradation

• Faster equilibration versus conventional silica phases

• Reduced sensitivity to mobile phase moisture (advantage in HILIC)

150 mm Length – High Resolution for Analytical HPLC

The 150 mm length provides increased resolving power for more complex separations.

• Higher resolution than 50 mm and 100 mm columns

• Improved separation of closely related or co‑eluting analytes

• Increased peak capacity for complex matrices

✅ Recommended for conventional HPLC workflows requiring high resolution and separation precision

✅ Particularly effective for ion exchange mode separations of small, highly polar compounds

Flow Rate & System Requirements (4.6 mm ID, 4 µm)

✅ Compatible with standard HPLC systems

• Typical flow rate range: ~1.0 – 1.5 mL/min

• Moderate system backpressure

• Compatible with UV and MS detectors

• Suitable for routine and advanced analytical workflows

✅ Best Practice: Use proper sample filtration and a matched guard column to maximize column lifetime

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Ideal for Complex Matrices

• Resolves co‑eluting compounds

• Effective for basic and positively charged analytes

pH‑Driven Selectivity Control

• WCX mechanism active at pH ≥ 6

• Retention tunable via pH and ionic strength

✅ Ideal when traditional reversed phase or ion exchange columns do not deliver adequate separation

TYPE‑C Silica Advantages

Extreme Robustness

• Stable under harsh conditions including very low pH

• Resistant to hydrolysis

Faster Equilibration

• Reduces method cycle times

No Strict Moisture Control (HILIC)

• Simplifies mobile phase handling

Extended Column Lifetimes

• Durable chemistry for routine and demanding analyses

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• RP, HILIC, and WCX in a single column

Standard HPLC Compatibility (4 µm)

• No need for UHPLC system

• Lower pressure than sub‑2 µm columns

High Resolution Capability

• Ideal for complex and difficult separations

Typical Applications

• Analytical HPLC method development

• Ion exchange separations of small polar compounds

• Complex matrix analysis

• Basic and positively charged analytes

• Pharmaceutical and bioanalytical workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Compatible with standard HPLC systems

• Suitable for both hydrophilic and hydrophobic analytes

• pH control is essential for optimizing WCX selectivity